Connor Wickham netted his first goal for the reserves as a young second string side lost 2-1 to Wycombe Wanderers at Marlow's Alfred Davis Ground on Tuesday evening.
The Chairboys went ahead via Matt Richards, a loanee from Derby rather than the out-of-favour Town man, in the 21st minute.
Wycombe added to their lead on 44 via trialist Simon Dawkins, a Spurs youngster.
Wickham pulled a goal back for the Blues when he scrambled the ball home in the 68th minute.
Overall, it was a lacklustre display from Chris Kiwomya's men, although better after the break. Liam Trotter was perhaps the pick of the players on show, although Jaime Peters had a strong second half.
Meanwhile, Watford's U18s defeated Southampton's youngsters 1-0 at St Mary's in the FA Youth Cup, lining up a possible fifth round tie with the Blues at Portman Road. Town's U18s take on Crystal Palace in their fourth round game at Portman Road next Tuesday (KO 7pm).
Reserves: Supple, Hyam, Ainsley, Watson, Harding, Peters, Reason, Upson, Trotter, Rhodes, Wickham (Murray 83). Unused: Ward, Cawley, Daniels, Eastman.