Tomas Holy, Janoi Donacien, Armando Dobra and Corrie Ndaba are among those starting for the Blues at Colchester this evening.
Holy is in goal with Donacien skippering from right-back and Matt Penney at left-back and Ndaba and Luke Woolfenden the centre-halves.
In midfield, 17-year-old Cameron Humphreys is again alongside Rekeem Harper with Lee Evans and Jon Nolan still absent.
Scott Fraser and Dobra look set for wide roles with Joe Pigott and Macauley Bonne up front.
Vaclav Hladky, Kane Vincent-Young, Toto Nsiala, Wes Burns and James Norwood, who all started on Saturday, are on the bench, as is Kayden Jackson, the only outfield player in the squad for the Crystal Palace game not to make it on to the field.
As expected, neither George Edmundson not Conor Chaplin are involved.
For Colchester, ex-Blues Dean Gerken, Luke Chambers, Tommy Smith, Frank Nouble, Alan Judge, Freddie Sears and Tom Eastman start with Cole Skuse on the bench.
Town’s allocation of 1,500 seats has sold out for the fixture, while the U’s are streaming the game for £10 here.
Colchester: Gerken, Clampin, Chambers, Smith (c), Wiredu, Nouble, Judge, Sears, Chilvers, Eastman, Coxe. Subs: Skuse, Hasanally, Thomas, Tovide, Terry, Cornish, Beadle, Trialist C, Trialist E, Trialist F.
Town: Holy, Donacien (c), Woolfenden, Ndaba, Penney, Harper, Humphreys, Dobra, Fraser, Pigott, Bonne. Subs: Hladky, Vincent-Young, Nsiala, Alexander, Healy, Burns, Norwood, Jackson, Clements. Referee: David Rock (Hertfordshire).